Equine Piroplasmosis Fluorescent Nucleic Acid Detection Kit
Equine protozoal myeloencephalitis (EPM) is a disease caused by single-celled parasites infecting the central nervous system (spinal cord and brain) of horses. It is primarily caused by Sarcocystis species, especially Sarcocystis neurona, and is an important cause of neurological disease in horses. AmpliSunTM Equine Piroplasmosis Fluorescent Nucleic Acid Test Kit uses the fluorescent PCR (real-time PCR) method to detect Sarcocystis neurona in samples such as cerebrospinal fluid and blood, providing an auxiliary tool for determining the etiology of the disease.
Product Parameters
| Production Name | AmpliSunTM Equine Piroplasmosis Fluorescent Nucleic Acid Detection Kit |
| Usage | Veterinary Diagnostics |
| Detection Method | Real-Time PCR |
| Specimen Type | cerebrospinal fluid, blood, spleen, tonsils, kidneys or lymph nodes |
| Storage Temperature | 2-30°C |
| Result Time | 45 Minutes |
| Packaging Specification | Individually sealed, 4 tests in total. |
| Format | Lyophilized reagent |
| Shelf Life | Up to Expiration Date Indicated on Package |
| Sensitivity | 500 copies/mL |
| Precision | ≤5% |
How to use？
Open the PCR tube and pipette 20 μL of extracted nucleic acid from the sample well of the nucleic acid extraction kit into the PCR tube. Close the cap tightly.
Mix the added liquid thoroughly with the solid in the PCR tube (vortex mixer is recommended) until no solid is visible (approximately 10 s). Then, briefly centrifuge for 5 s using a mini centrifuge to ensure all liquid is at the bottom of the PCR tube and no bubbles remain. Finally, place the tube into the PCR instrument for detection.
Materials provided
8 sets of PCR amplification freeze-dried reagents
8 sets of nucleic acid extraction reagents
8 sampling swabs
8 vials of sample preservation solution
