SunshinyMed Tech Co. Ltd

Porcine Ileitis Antibody ELISA Kit

This ELISA kit is designed for the detection of antibodies against Lawsonia intracellularis in swine serum samples. The assay has been successfully used worldwide to determine the epidemiological status of farms and to support the appropriate planning of prevention and control measures.
Description Package Information Inquiry

The lesions Porcine Proliferative Enteropathy (PPE), also known as ileitis, were first described in pigs in Ames, IA by Biester and others in 1931. The cause of PPE is Lawsonia intracellularis an obligately intracellular bacterium. It grows in the cytoplasm of intestinal epithelial cells and causes the thickening of the mucosa and colon. Infection of pigs with this bacterium is consistently linked with the presence of proliferative lesions of the mucosa of the ileum and large intestine. The disease has a worldwide distribution and occurs commonly in all pig raising regions and in all farm management systems.


Lawsonia intracellularis does not grow on conventional bacterial media and requires tissue culture under reduced oxygen tension. However, serological diagnosis of PPE is commonly achieved by means of tests such as indirect immunoflourescence assays (IFA) or immuno-peroxidase monolayer assays (IPMA), both of which involve cumbersome procedures to culture the organism in-vitro. ELISA tests are advantageous in that they are easy to perform and do not require culture of the organism.



Product Information

【Product Name】

  Porcine Ileitis Antibody ELISA Kit.

【Specification】96T/Box.

【storage Conditions】2~8'C.

【Shelf Life】6 months, Avoid repeated freeze-thaw cycles.

【Samples】Serum.

【Intended Use】

  For the detection of ileitis antibodies in porcine serum.


Product Features

Standardized Protocols

  • Pre-coated plates andcalibrators minimizehuman error and enhanceresult comparability.

Automation Compatibility

  • Compatible withmicroplate readers andlaboratory automationsystems.

Simplified Workflow

  • Requires no specializedinstruments, suitable forrapid screening inveterinary clinics or farms

High Reproducibility

  • Stable productionprocesses ensureconsistent performanceacross reagent batches.


01-Positive Control  500μl/tube×1
02-Negative Control  500μl/tube×1
03-10×Wash Buffer50ml/bottle×1
04-Enzyme-labeled Antibody150μl/bottle×1
05-Enzyme-labeled Antibody Diluent11ml/bottle×1
06-Sample diluent40ml/bottle×1
07-Substrate Solution11ml/bottle×1
08-Stop Solution6ml/bottle×1
09-Instructions1 serving