
Porcine Ileitis Antibody ELISA Kit
The lesions Porcine Proliferative Enteropathy (PPE), also known as ileitis, were first described in pigs in Ames, IA by Biester and others in 1931. The cause of PPE is Lawsonia intracellularis an obligately intracellular bacterium. It grows in the cytoplasm of intestinal epithelial cells and causes the thickening of the mucosa and colon. Infection of pigs with this bacterium is consistently linked with the presence of proliferative lesions of the mucosa of the ileum and large intestine. The disease has a worldwide distribution and occurs commonly in all pig raising regions and in all farm management systems.
Lawsonia intracellularis does not grow on conventional bacterial media and requires tissue culture under reduced oxygen tension. However, serological diagnosis of PPE is commonly achieved by means of tests such as indirect immunoflourescence assays (IFA) or immuno-peroxidase monolayer assays (IPMA), both of which involve cumbersome procedures to culture the organism in-vitro. ELISA tests are advantageous in that they are easy to perform and do not require culture of the organism.
Product Information
【Product Name】
Porcine Ileitis Antibody ELISA Kit.
【Specification】96T/Box.
【storage Conditions】2~8'C.
【Shelf Life】6 months, Avoid repeated freeze-thaw cycles.
【Samples】Serum.
【Intended Use】
For the detection of ileitis antibodies in porcine serum.
Product Features
Standardized Protocols
Pre-coated plates andcalibrators minimizehuman error and enhanceresult comparability.
Automation Compatibility
Compatible withmicroplate readers andlaboratory automationsystems.
Simplified Workflow
Requires no specializedinstruments, suitable forrapid screening inveterinary clinics or farms
High Reproducibility
Stable productionprocesses ensureconsistent performanceacross reagent batches.
| 01-Positive Control | 500μl/tube×1 |
| 02-Negative Control | 500μl/tube×1 |
| 03-10×Wash Buffer | 50ml/bottle×1 |
| 04-Enzyme-labeled Antibody | 150μl/bottle×1 |
| 05-Enzyme-labeled Antibody Diluent | 11ml/bottle×1 |
| 06-Sample diluent | 40ml/bottle×1 |
| 07-Substrate Solution | 11ml/bottle×1 |
| 08-Stop Solution | 6ml/bottle×1 |
| 09-Instructions | 1 serving |
