Introduction

Chicken Infectious Anemia (CIA) is an important viral disease of poultry caused by Chicken Infectious Anemia Virus (CIAV). It primarily affects young chicks, especially those within the first few weeks of life. CIAV is a small, non-enveloped, single-stranded DNA virus belonging to the genus Gyrovirus. It has strong immunosuppressive effects and may cause aplastic anemia, generalized lymphoid atrophy, and impaired immune function in infected flocks.

Chicks infected with CIAV commonly exhibit pale combs, wattles, and mucous membranes, depression, growth retardation, reduced feed conversion efficiency, and increased mortality. Due to the marked immunosuppression induced by the virus, affected birds are more susceptible to secondary bacterial, viral, or fungal infections, which may further aggravate clinical signs and increase economic losses for poultry producers. CIAV infection may also interfere with vaccine responses, creating additional challenges for flock health management and disease control.

This kit is designed with specific primers and fluorescent probes targeting conserved regions of the CIAV genome, enabling rapid, sensitive, and specific detection of CIAV nucleic acids in samples. It is suitable for the auxiliary diagnosis of CIAV infection, epidemiological investigations, breeder flock monitoring and purification programs, as well as routine disease surveillance in poultry farms.

Product Features

• Rapid Detection

      Results are available within 60 minutes.

• Convenient Operation

      Pre-mixed reagents are ready to use. Simply add extracted samples or controls without additional reaction preparation steps.

• Broad Sample Applicability

     Compatible with nucleic acids extracted from a variety of tissue, body fluid, blood, and excreta samples collected from live or dead animals.

• Easy Result Interpretation

      Results can be readily interpreted based on Ct values.

• Wide Instrument Compatibility

   Compatible with ABI 7500, Agilent MX3000P/MX3005P, LightCycler, Bio-Rad, Eppendorf, and other real-time fluorescent quantitative PCR instruments.

Note: For some products, the exogenous internal control is combined with the PCR Master Mix in a single tube.