Introduction

The Haemophilus paragallinarum (HPG) Nucleic Acid Detection Kit (Fluorescent PCR Method) is designed for the rapid detection of nucleic acids associated with infectious coryza in chickens. Infectious coryza is primarily caused by Haemophilus paragallinarum infection and may result in nasal discharge, conjunctivitis, infraorbital sinus swelling, facial edema, reduced feed intake, and decreased egg production and hatchability in laying hens. The disease can spread rapidly within flocks, while clinically affected birds and asymptomatic carriers may both serve as sources of infection, contributing to persistent and recurrent outbreaks.

This kit uses fluorescent PCR technology to detect HPG-specific nucleic acid sequences and is suitable for the auxiliary diagnosis of suspected cases, flock screening, and carrier surveillance. Testing samples such as nasal swabs and infraorbital sinus exudates from affected birds can help identify HPG infection at an early stage and support differential diagnosis from other diseases with similar respiratory signs or facial swelling, including infectious laryngotracheitis, Klebsiella infection, and swollen head syndrome. The results provide reliable support for flock isolation, treatment, disease control, eradication programs, and biosecurity management.

Product Features

• Rapid Detection

      Results are available within 60 minutes.

• Convenient Operation

      Pre-mixed reagents are ready to use. Simply add extracted samples or controls without additional reaction preparation steps.

• Broad Sample Applicability

     Compatible with nucleic acids extracted from a variety of tissue, body fluid, blood, and excreta samples collected from live or dead animals.

• Easy Result Interpretation

      Results can be readily interpreted based on Ct values.

• Wide Instrument Compatibility

   Compatible with ABI 7500, Agilent MX3000P/MX3005P, LightCycler, Bio-Rad, Eppendorf, and other real-time fluorescent quantitative PCR instruments.

Note: For some products, the exogenous internal control is combined with the PCR Master Mix in a single tube.