Introduction

Aquaculture has become an important economic industry worldwide. With the increase in farming density and cross-regional trade, the demand for prevention and control of bacterial diseases in aquatic animals continues to grow. Streptococcus iniae，also known as S. shiloi, is an important Gram-positive bacterial pathogen of fish. It was first discovered in captive Amazon river dolphins and was later confirmed to infect a variety of economically important fish species, causing streptococcosis outbreaks worldwide.

Streptococcus iniae infection can lead to septicemia in fish. Common clinical signs include abnormal swimming behavior, loss of balance, unilateral or bilateral exophthalmia, cloudy eyes, ascites, hemorrhage in internal organs and at the base of fins, darkening of the skin, pale liver, and enlarged spleen. This pathogen has been reported in various aquaculture species, including Japanese flounder, red porgy, Nile tilapia, mandarin fish, golden pompano, and Adriatic sturgeon. Severe infections may result in high mortality and significant economic losses. In recent years, S. iniae has also been recognized as a potential zoonotic pathogen. Therefore, rapid and accurate detection of this bacterium is of great importance for disease monitoring, early diagnosis, and biosecurity management in aquaculture.

SunshinyMed Streptococcus iniae Detection Kit is designed for the detection of Streptococcus iniae in fish samples, providing a rapid, sensitive, and specific testing solution for aquaculture farms, veterinary laboratories, aquatic animal disease diagnostic institutions, and research organizations. The kit is suitable for pathogen confirmation in suspected streptococcal infections, health monitoring of cultured fish populations, epidemiological investigations, and evaluation of treatment and prevention measures.

Product Features

• Rapid Detection

      Results are available within 60 minutes.

• Convenient Operation

      Pre-mixed reagents are ready to use. Simply add extracted samples or controls without additional reaction preparation steps.

• Broad Sample Applicability

     Compatible with nucleic acids extracted from a variety of tissue, body fluid, blood, and excreta samples collected from live or dead animals.

• Easy Result Interpretation

      Results can be readily interpreted based on Ct values.

• Wide Instrument Compatibility

   Compatible with ABI 7500, Agilent MX3000P/MX3005P, LightCycler, Bio-Rad, Eppendorf, and other real-time fluorescent quantitative PCR instruments.

Note: For some products, the exogenous internal control is combined with the PCR Master Mix in a single tube.