Introduction

Streptococcus agalactiae is a common and highly harmful bacterial pathogen in tilapia aquaculture. It can cause streptococcosis, leading to symptoms such as reduced appetite, abnormal swimming behavior, cloudy eyes, eye swelling, and exophthalmia. The disease is highly contagious and associated with high mortality, especially under stressful conditions such as high-density farming, temperature fluctuations, and hypoxia. It can also spread horizontally through feed and infected fish, causing significant economic losses to the tilapia aquaculture industry.

Traditional bacterial culture methods are time-consuming and have limited sensitivity, making them difficult to meet the needs of early screening and rapid disease control. SunshinyMed Streptococcus agalactiae Detection Kit adopts Real-time PCR fluorescence quantitative detection technology and provides PCR Master Mix, specific Primer Mix, and an Internal Control to monitor possible PCR inhibition or sample preparation failure.

This kit enables rapid, sensitive, and specific detection of Streptococcus agalactiae nucleic acid. It is suitable for pathogen screening, auxiliary diagnosis, disease monitoring, and laboratory research using tilapia and other aquatic animal samples, as well as aquaculture environmental samples. It provides a reliable molecular detection tool for the early detection and control of tilapia streptococcosis.

Product Features

• Rapid Detection

      Results are available within 60 minutes.

• Convenient Operation

      Pre-mixed reagents are ready to use. Simply add extracted samples or controls without additional reaction preparation steps.

• Broad Sample Applicability

     Compatible with nucleic acids extracted from a variety of tissue, body fluid, blood, and excreta samples collected from live or dead animals.

• Easy Result Interpretation

      Results can be readily interpreted based on Ct values.

• Wide Instrument Compatibility

   Compatible with ABI 7500, Agilent MX3000P/MX3005P, LightCycler, Bio-Rad, Eppendorf, and other real-time fluorescent quantitative PCR instruments.

Note: For some products, the exogenous internal control is combined with the PCR Master Mix in a single tube.